Composite

Part:BBa_K5349007:Design

Designed by: Tomoharu Sakata   Group: iGEM24_BotchanLab-Tokyo   (2024-09-26)


Sleeping Beauty Mutase operon


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2926
    Illegal BglII site found at 5197
    Illegal BamHI site found at 1030
    Illegal BamHI site found at 1226
    Illegal BamHI site found at 3363
    Illegal XhoI site found at 5901
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 560
    Illegal SapI.rc site found at 3822


Design Notes

For cloning, overlap sequences were designed for each fragment, and the assembly was performed using NEBuilder. Each fragment was synthesized using Integrated DNA Technologies' DNA synthesis service.


Source

  • promotor:
  • RBS:[ B0034]
  • Sbm operon
  • sfGFP
  • terminator

References